| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Horning, Caitlin L (8)
|
MADST |
39.54 |
36.20 |
-3.34 |
| 2 |
Kolessar, Kaitlyn R (7)
|
MADST |
40.98 |
39.12 |
-1.86 |
| 3 |
Beyah, MeKhai (8)
|
MASAKA |
40.84 |
40.02 |
-0.82 |
| 4 |
Phillips, Victoria L (8)
|
MADST |
39.26 |
40.22 |
0.96 |
| 5 |
Ryan, Maxalyn E (6)
|
MADST |
45.77 |
47.17 |
1.40 |
| 6 |
Radziewicz, Gabriella E (7)
|
MADST |
48.59 |
50.10 |
1.51 |
| 7 |
O'Connor, Maeve O (8)
|
MASAKA |
53.78 |
50.90 |
-2.88 |
| 8 |
Williams, Paige (8)
|
MASAKA |
50.93 |
52.05 |
1.12 |
| 9 |
Kleinmeulman, Cayla E (8)
|
MADST |
50.00 |
52.94 |
2.94 |
| 10 |
Hung, Danielle Hana T (8)
|
MAACST |
|
54.24 |
- |
| 11 |
Ovalle, Mariana (8)
|
MAACST |
|
59.30 |
- |
| 12 |
Baraka, Zorah A (8)
|
MASAKA |
|
1:20.69 |
- |
|
Alamo-Rosas, Victoria L (7)
|
MASAKA |
1:17.20 |
DQ |
- |
|
Williams, Victoria A (6)
|
MASAKA |
|
NS |
- |